MutS‐Homolog2 silencing generates tetraploid meiocytes in tomato ( Solanum lycopersicum )

MutS-Homolog2 silencing generates tetraploid meiocytes in tomato (Solanum lycopersicum)

Pre-meiotic endomitosis in the cytokinesis-defective tomato mutant pmcd1 generates tetraploid meiocytes and diploid gametes.

Sexual polyploidization through the formation and functioning of 2n gametes is considered a major route for plant speciation and diversification. The cellular mechanism underlying 2n gamete formation mostly involves a restitution of the meiotic cell cycle, generating dyads and triads instead of tetrad meiotic end-products. As an alternative mechanism, the tomato mutant pmcd1 (for pre-meiotic cy...

RNA interference as a gene silencing tool to control Tuta absoluta in tomato (Solanum lycopersicum)

RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential inse...

In-Vitro Organogenesis in Tomato (Solanum Lycopersicum) Using Kinetin

This research was undertaken for optimizing simple and reproducible protocol on indirect regeneration in tomato using kinetin. The effect of kinetin for efficient callus production was studied and its results were interpreted. Hypocotyls and cotyledons were used as explants and effect on callus derived regeneration was greatly influenced by addition of kinetin in media. The individually positio...

Genetic Diversity in Tomato (Solanum lycopersicum) and Its Wild Relatives

Tomato, ranking 1st in the world for vegetables, accounts for 14% of world vegetable production (over 100 million metric tons/year $ 1.6 billion market; (Food and Agriculture Organisation [FAO] 2010). Tomato is a rich source of micronutrients for human diet. It is also an acknowledged model species for research on fruit development and metabolite accumulation. The major goals of tomato breeders...